Electrochemical and biological characterization of Ti

Mar 29, 2024

Scientific Reports volume 13, Article number: 2312 (2023) Cite this article

1207 Accesses

9 Citations

4 Altmetric

Metrics details

The performance of current biomedical titanium alloys is limited by inflammatory and severe inflammatory conditions after implantation. In this study, a novel Ti–Nb–Zr–Si (TNZS) alloy was developed and compared with commercially pure titanium, and Ti–6Al–4V alloy. Electrochemical parameters of specimens were monitored during 1 h and 12 h immersion in phosphate buffered saline (PBS) as a normal, PBS/hydrogen peroxide (H2O2) as an inflammatory, and PBS/H2O2/albumin/lactate as a severe inflammatory media. The results showed an effect of the H2O2 in inflammatory condition and the synergistic behavior of H2O2, albumin, and lactate in severe inflammatory condition towards decreasing the corrosion resistance of titanium biomaterials. Electrochemical tests revealed a superior corrosion resistance of the TNZS in all conditions due to the presence of silicide phases. The developed TNZS was tested for subsequent cell culture investigation to understand its biocompatibility nature. It exhibited favorable cell-materials interactions in vitro compared with Ti–6Al–4V. The results suggest that TNZS alloy might be a competitive biomaterial for orthopedic applications.

The demand for artificial implants is growing as the elderly population is growing up in the various countries increases1. Approximately 80% of commercial implant devices are made of metallic biomaterials for correcting deformities in the skeletal system2,3. Commercially pure titanium (CP-Ti, Grade 2, UNS R50400) is conventionally used as metallic biomaterials in dental devices, joint orthopedic replacements, and cardiovascular stents because of its proper corrosion resistance and satisfactory biocompatibility4. CP-Ti does not have all desirable properties for biomedical implants, so Ti–6Al–4V alloy (also known as Grade 5 alloy, UNS R56400) with higher strength is also most widely used5. However, there is a growing concern on corrosion and corresponding elution of aluminum and vanadium that have toxic and side effects on long-term applications6,7 and newer improved titanium alloys with lower elastic modulus, higher strength, and superior biological performance are being developed7,8.

One of the developed alloys without aluminum and vanadium is based on the Ti–Nb–Zr system (UNS R58130 by ASTM F1713) has more biocompatible Nb and Zr elements, and lower Young’s modulus in a range of 60–80 GPa9,10,11. Combining Nb and Zr in this alloy resulted in a near β-Ti phase structure more corrosion resistant than α-Ti and β-Ti phases but it did not have achieved a desired level of biocorrosion resistance without compromising its reactivity to the cells12. Among other possible additives silicon has been considered a vital element in the human body for biological reactions, providing a driving force for the growth and development of new bone and connective tissues13,14.

After a biomaterial implantation, the body’s immune system is activated to protect the host against infections and tissue damage, additionally complicated with a foreign body reaction15,16 and surface deposition of various biomolecules and proteins such as albumin17. Leukocytes increase their consumption of oxygen by using the respiratory burst to produce reactive oxygen species (ROS), lactic acid, including hydrogen peroxide (H2O2) and its derivatives, and hypochlorous acid (HOCl) into the extracellular medium16,18,19,20. For bone tissue, osteoclasts also express HCl which together creates an oxidative acidic environment with pH decrease from neutral to 5 and lower during inflammation18. In the severe inflammatory condition, neutrophils, macrophages, and microorganisms can create a very oxidative and acidic medium with pH ⁓ 2–3 surroundings the implant, even sufficient to dissolve very resistant materials such as gold21. The passive protective film on titanium in these conditions start to undergo degradation, accelerating corrosion rate and causing surface roughening and formation of intensely hydrated porous TiOOH compound22,23,24,25. Recent studies showed that the combination of albumin and H2O2 accelerated the corrosion rate of CP-Ti and Ti–6Al–4V26,27 and the presence of lactic acid and H2O2 decreased the corrosion resistance considerably28,29. As a result of the complicated in vivo environment, corrosion of Ti implants can be considerably accelerated, resulting in a significantly shorter implant life and higher risk of failures30,31. Therefore, proper alloying additives to high-performance titanium alloys are required to prevent implant failures in inflammatory conditions. Silicon has been introduced to some Ti-based alloys32,33,34, but it has not yet been sufficiently analyzed when added to the Ti–Nb–Zr system. So, there is an interest to assess as Ti–Nb–Zr–Si alloy for potential material for orthopaedic applications.

In this work, a novel Ti–Nb–Zr–Si (TNZS) alloy was made and its in vitro characteristics including the corrosion performance and biological response to cell cultures were evaluated. Severe inflammatory conditions for electrochemical corrosion characterization were simulated by incorporating of H2O2, bovine serum albumin (BSA), HCl and calcium l-lactate hydrate (CLH) into the normal physiological solution (corrosion behavior of titanium was previously studied when albumin and lactic acid are added to H2O2-containing solutions separately26,27,28,29,35,36,37,38,39,40, but not yet when both are added simultaneously). To the best of authors’ knowledge, the in vitro corrosion of CP-Ti, Ti–6Al–4V, and especially TNZS has never been reported in the severe inflammatory conditions. The electrochemical corrosion measurements of TNZS were conducted in the inflammatory and severe inflammatory conditions simulated by the addition of H2O2, and H2O2/BSA/CLH to the acidified phosphate-buffered saline (PBS), respectively. These in vitro characteristics of TNZS alloy as potential orthopedic biomaterials are discussed and compared with conventionally used CP-Ti and Ti–6Al–4Valloys.

The optical microscopy (Fig. 1a) and SEM (Fig. 1b) images of TNZS alloy clearly show consisting lamellar eutectics discontinuously distributed on the matrix. Figure 1c shows a typical XRD pattern of the TNZS alloy at α-Ti, β-Ti, and intermetallic silicide (S1 of the M5Si3 type and S2 of M2Si type) phases were identified and indexed well as marked at the diffractograms. The EDS analysis was carried out on six points on TNZS microstructure at the different regions in the precipitated phase and matrix to examine the elemental distribution as presented in supplementary Table ST1 (Supplementary Materials data file), exhibiting a clear elements partition between these phases. The metallic matrix has ratio Nb:Zr = 2:1 whereas in the silicides it is the opposite Nb:Zr = 1:2, indicating that addition of silicon to Ti–Nb–Zr alloy and subsequent formation of silicides leads to higher binding of zirconium in the intermetallic phases leaving niobium to enrich metallic phase34,41.

(a) OM image, (b) back-scattered (BSE) SEM micrograph, (c) XRD pattern, and (d) magnified BSE-SEM micrograph with EDS point analyses of TNZS alloy.

Thermodynamic calculations results show that (Supplementary Fig. S1a) during solidification primary ß-phase is forming first following precipitation of M5Si3 silicide (S1) as the major intermetallic phase and subsequent α-ß transus of the metallic solid solution phase. The M2Si (S2) phase can only form at local areas where zirconium concentration is higher (Supplementary Fig. S1b), as this phase does not form in the binary Ti–Si or Nb–Si systems. Enrichment of metallic phase with niobium might be seen beneficial from the point of corrosion resistance of this alloy. One can also expect that if the phase transformations are following the diagram (Supplementary Fig. S1a) than S1 silicides will precipitate in the ß-rich matrix providing additional strengthening against deformation. This requires an additional analysis as the mechanical properties of the TNZS alloy are not considered in this study, but it indicates a possibility of extra options for the alloy properties modification via heat treatment.

Figure 2 shows the OCP evolution of specimens after 1 and 12 h of immersion in normal, inflammatory, and severe inflammatory conditions media. The OCP values increased with immersion time in all conditions except CP-Ti sample in the severe inflammatory condition. This indicates that oxide layers have thickened or reconstructed in terms of their structure or chemical composition, which can be the result of the suppression of anodic reactions and a decrease in the anodic current density42. Under all conditions, TNZS alloy has shown a nobler potential compared with CP-Ti and Ti–6Al–4V, presumably due to formation of a passive layer consisting of more niobium oxide, also denser and defect-less than oxide layers usually forming on CP-Ti and Ti–6Al–4V43,44. Observed fluctuations in the OCP of the TNZS alloy specimens in the inflammatory environment can be caused by the high resistance of the passive layer, which may become broken and then to repassivated in the presence of hydrogen peroxide.

OCP monitoring of the CP-Ti, Ti–6Al–4V, and TNZS specimens exposed in (a) simulated normal, (b) inflammatory, and (c) severe inflammatory conditions for 1 h and 12 h.

As seen in Fig. 2a,b, when H2O2 is added to PBS (to simulate the inflammatory conditions), the OCP values shift to more electropositive values. This can be explained by H2O2 decomposition to H2 and O2, acting as an extra cathodic reaction to the oxygen reduction43. Moreover, Tengvall’s studies suggest that the positive OCP in H2O2-containing solutions are often caused by intermediates with a strong oxidizing character adsorbing to the passive film on Ti, such as HO2 and HO44,45. In contrast, in a severe inflammatory solution containing BSA and CLH in addition to H2O2 in PBS (Fig. 2c), the OCP of all specimens was consistently lower than that in the presence of PBS and H2O2 alone (inflammatory), but higher than in PBS alone (normal media). Recent studies suggested that BSA adsorbs on titanium surfaces by electrostatic interactions or chemisorption through amino/carboxylate groups, decreasing the rate of cathodic reduction of H2O246,47,48. The same behavior has been reported in the addition of the CLH to the H2O2-containing solutions, which significantly blocks the cathodic site by adsorption on the surface, resulting in lower values of the OCP28,29. This does however not mean that the BSA and CLH inhibit the corrosion of Ti in the severe inflammatory medium and it seems necessary to perform PDP and EIS tests for further electrochemical studies.

Figure S2a–i in the Supplementary Data file represent the Nyquist, Bode modulus and Bode phase plots of EIS tests, respectively. The symbols are the experimental data and the solid lines are fitting data. The equivalent circuit (Supplementary Fig. S3) based on a single time constant on Bode plots was selected to represent the electrochemical corrosion phenomenon in normal, inflammatory and severe inflammatory condition, which contains the solution’s resistance (Rs), constant phase element (CPE), and passive layer’s resistance (Rpl)49. Some heterogeneities, such as surface roughness, impurities, or defects, made it necessary to introduce a CPE element instead of a simple capacitor48. The CPE element impedance (Ohm·cm-2) can be described by Q and n parameters through following equation49:

where n is the power coefficient varied between 0 and 1, ω is an angular frequency, and \(j= \sqrt{-1}\). Interfacial capacitance is usually approximated by the value of Q when n → 1, but this approach has been found to be inaccurate49. The following Eq. (2) is used to calculate the effective capacitance (Ceff) values of interfacial capacitance so that a more realistic value can be assigned50, which has been shown more accurate:

A semiconductive oxide film thickness value (deff) formed on titanium alloys can be evaluated using this equation51 as:

where ε is the layer’s dielectric constant (taken as 45 for pure Ti and its alloys52), and ε0 is the vacuum permittivity (8.85 × 10−14 F·cm−1). The fitted and calculated values through Eqs. (1)–(3) are listed in Supplementary Table ST2. The Rpl values of all specimens increased after 12 h in all solutions, suggesting the formation and growth of the passive protective film. In inflammatory media the concentration of H2O2 is decreasing with time due to the decomposition of H2O2 to H2O and O243,53 and here the reduction of the media oxidative power facilitates the self-healing of the passive layer. It can be seen from Supplementary Table ST2 that the largest Rpl values among the specimens were obtained for the TNZS alloy.

When comparing the resistances of passive layers using the designated Rpl values, the inflammatory condition has a destructive effect on the layers and caused a decrease in passive layers’ resistance. This is due to the formation of Ti(IV)-H2O2 complex compounds in response to the reaction between Ti and H2O254. The corrosion resistance in the severe inflammatory conditions has noticeably deteriorated because of the co-presence of BSA, CLH, and H2O2 where Rpl values show a synergetic effect on both BSA and CLH on increase of the degradation of titanium alloys. It was claimed that the high oxidation potential of ROS intermediates in the presence of biomolecules produces a strong electric field across the passive layer, leading to an acceleration in the oxidation process of Ti55,56. Another possible explanation for decreased Rpl in severe inflammatory condition is due formation of a lactate-chelating compound when titanium reacts with CLH57,58. Similar behavior in Ti was observed when exposed to lactic acid in artificial saliva57.

Bode phase plots (Fig. S2i) show that TNZS alloy immersed in the severe inflammatory media exhibited phase angles close to 80°, confirming its excellent corrosion resistance and slower reactivity compared to other specimens with phase angles of 50°. This indicates that the microstructure and chemical composition determines the level of protection, governed by the passive oxide layers formed on TNZS. Calculated Ceff values are within a range of 10–5 F·cm−2 (Supplementary Table ST2), which is typical of what has been reported for bulk Ti and its alloys in simulated body fluids59,60. Calculated values of passive layer thickness (deff) did not exceed a few nanometers, which is comparable to values reported by other electrochemical and non-electrochemical studies on Ti surfaces61.

Supplementary Figure S4 illustrates PDP curves recorded after 1 and 12 h immersion in normal, inflammatory and severe inflammatory conditions for all materials. Here the corrosion behavior of specimens is being evaluated by the corrosion potential (Ecorr), corrosion current density (icorr), and polarization resistance (Rp). The latter could be calculated by the Stern–Geary Equation38:

where βa and βc are respectively Tafel slopes of anodic and cathodic branches on the PDP curve. The Ecorr, icorr, and βa and βc values were determined by the Tafel extrapolation method and reported in Supplementary Table ST3. The typical passivation behavior of Ti-based materials may be responsible for the absence of Tafel behavior in anodic branches under normal conditions, thus making it impossible to determine the βa, and only βc values are reported62. The plotted icorr vs. Ecorr, and Rp vs. Ecorr are shown in Fig. 3.

icorr vs. Ecorr: (a) 1 h and (b) 12 h; Rp vs. Ecorr: (c) 1 h and (d) 12 h.

It is seen that in all conditions icorr of specimens has decreased after 12 h immersion. For the normal media this can be associated with the formation of a stable surface oxide film protecting surfaces from further attack. The increased corrosion resistance in inflammatory and severe inflammatory conditions can be attributed to the catalytic decomposition of H2O2 to H2O and O2 during the 12 h immersions decrease the oxidizing power of the solutions43,53. From anodic branches of PDP curves it is seen that specimens had passive behavior in the normal media but not in the inflammatory and severe inflammatory conditions23,27,29. As seen in Fig. 3a,b, addition of H2O2 to the PBS has lead to increase of icorr values for all specimens, indicating a higher corrosion rate in the inflammatory conditions. These results are consistent with previous studies of increased icorr and decreased Rp of CP-Ti and Ti–6Al–4V in the H2O2-containing biological solutions. Similar to the OCP shown in Fig. 2b, the Ecorr values were electropositive for the inflammatory condition (H2O2-containing PBS) compared to the normal condition. In the presence of BSA, CLH and H2O2 in PBS (severe inflammatory media), icorr increases (Fig. 3a,b) and Rp decreases (Fig. 3c,d) drastically, suggesting that BSA and CLH along with H2O2 have a synergetic effect on the corrosion behavior of Ti implants. Some fluctuations can be seen on the branches of PDP curves in Fig. S4 in severe inflammatory condition are attributed to the effect of the adsorbed biomolecules on the titanium surface on anodic and cathodic reactions in this condition. Similar fluctuations in the PDP curves were previously observed in the corrosion studies of stainless steel in bacteria-containing solutions63.

For CP-Ti material a gradual increase in icorr was observed in inflammatory and severe inflammatory conditions, suggesting the CP-Ti surface becomes oxidized in the presence of H2O2, but the formed oxide is dissolved faster than needed to provide proper protection64. Ti–6Al–4V alloy showed better corrosion performance than CP-Ti in all conditions which is consistent with the literature65. There the lowest corrosion resistance was observed in inflammatory media compared with the normal condition. It has been suggested that the increase in the icorr in this condition is caused by the dissolution of vanadium oxide on the surface of Ti–6Al–4V alloy, and then the generation of vacancies in the oxide layers, further enhanced by H2O2 and Cl- ions46,65. In terms of icorr, TNZS alloy showed the lowest values between all specimens in all media and immersion times, and it also had less fluctuations than for Ti–6Al–4V alloy. It was discussed that more fluctuations of current observed in the anodic branches are related to the surface roughening and continuous formation/destruction of metastable pits on the electrode surface54,66, indicating that the passive layers on Ti–6Al–4V are less stable in this medium.

The highest corrosion resistance of TNZS can be mainly attributed to the addition of especially Nb, and to lesser extent to Zr and Si elements in the alloy, resulting in the formation of a more inert and stable oxide film. Other studies showed that a ternary Ti–Nb–Zr alloy has improved stability of the surface films exhibiting exceptionally high resistance to oxidizing acids39,43. Some reports showed the role of Si in the stabilizing the crystallized phases in Si–Nb-based alloys that might be helpful to promote corrosion resistance67,68,69,70. It has been also reported that the addition of Si can improve the corrosion resistance of Ti alloys in acidic solutions due to the formation of stable SiO2 and Si-doped TiO2 passive layers on the Ti–Si alloy34,71. The impressive corrosion performance of TNZS alloy in inflammatory and severe inflammatory conditions is due to the presence of Nb-enriched α + β matrix and S1/S2 intermetallic phases. Supplementary Table ST4 compares the corrosion parameters extracted from PDP curves of the TNZS alloy fabricated in this study with Ti–Nb–Zr–Me (Me = metal) alloys reported in the literature, showing that TNZS alloy is less susceptible to corrosion than CP-Ti, Ti–6Al–4 V, and Ti–Nb–Zr–Me72,73,74,75,76 alloys when exposed to similar conditions.

Figure 4 represents the top-view SEM images of specimens after 12 h immersion and recording the corresponding PDP curves in each simulated solution. The EDS elemental analysis results of specified areas in Fig. 4 are listed in Supplementary Table ST5. There are obvious differences in the surface morphologies between CP-Ti, Ti–6Al–4V, and TNZS specimens in normal, inflammatory, and severe inflammatory conditions. As seen in Fig. 4a–c, the inferior pitting corrosion and surface roughening occurred for CP-Ti, in all simulated conditions. The decrease in the amount of Ti and the increase in the amount of oxygen in inflammatory and severe inflammatory conditions indicate the promotion of the implant’s degradation rate and growth of the oxide layer over time for these environments compared with the normal condition.

Top-surface SEM micrographs of specimens exposed in simulated normal, inflammatory and severe inflammatory conditions for 12 h: (a–c) CP-Ti, (d–f) Ti–6Al–4V, and (g–i) TNZS.

In the micrographs of the TNZS alloy exposed in the normal solution (Fig. 4g), there are fewer pits and localized corrosion products, and the polished surface with scratches can still be seen. Figure 4e shows the mud-crack morphology and large pits with an increase in Cl content on the surface of Ti–6Al–4V, suggesting a higher corrosion rate in acidic H2O2-containing PBS solution. The same morphologies were reported for prolonged exposure to Ti–6Al–4V in inflammatory conditions23. The adsorption of Cl- ions to oxygen vacancies in the presence of H2O2 on the surface of the oxide/solution induces a flux of cation vacancies to the metal oxide interface and their annihilation77,78. As shown in Table ST5 for regions C, F, and I, more carbon is present correlating with expected BSA and CLH adsorption on Ti surfaces when immersed in the severe inflammatory condition media29,79. These morphological changes in the different conditions are correlated with the variation of n values in Eq. (1), as their reduction in inflammatory and severe inflammatory conditions (Table ST2) is indicative of larger surface heterogeneity in these conditions (Fig. 4).

It is evident from Fig. 4f that intergranular voids formed through the dissolution of some material are leading to the degradation of the Ti–6Al–4V surface in the severe inflammatory conditions. It is known that the pitting resistance of Ti alloys can be significantly reduced by the addition of Al and V elements and several studies have reported corrosion of Ti–6Al–4V alloy in the presence of H2O2 due to vanadium oxide dissolution77. Compared with CP-Ti and Ti–6Al–4V materials TNZS showed lower localized corrosion damage (Fig. 4h,i) in inflammatory and severe inflammatory conditions, but small pits and some corrosion products nevertheless have appeared on the surface. The silicide phases can also play a crucial role in the corrosion behavior of the TNZS in acidic H2O2-containing solutions as recent studies shown e.g. Ti5Si3 (equivalent to S1 phase in the present study) had excellent corrosion resistance in acidified H2O2 solution80. Hence it can be concluded that the impressive corrosion performance of TNZS alloy in inflammatory and severe inflammatory conditions is also enhanced by the presence of silicide intermetallic phases.

The results of the cells-materials interactions are shown in Fig. 5 and Supplementary Figs. S5–S7. For HOC (Fig. 5) it can be visually seen that cells on TNZS are doing much better than on Ti–6Al–4V alloy (with the same surface morphology) although lower than at the negative control. Nevertheless, the HOC coverage area (%) on TNZS remains high enough at 40–45% for days 9 to 27 vs. negative control (40–50%) and especially Ti–6Al–4V (15–25% only). For HUVEC (Fig. S5) this difference is clearly favorite for TNZS (20–25% in day 15) vs. negative control (10%) and Ti–6Al–4V (< 2% only).

HOC proliferation and growth (× 100) of negative control, Ti–6Al–4V, and TNZS at 1, 3, 9, 15, and 27 days.

In gene expression analysis for HOC, much higher levels of ALP and ColI are seen for TNZS, and these differences are stable in time (Fig. S6a,b). At the first day, Cbfa is well expressed on TNZS indicating potentially high osteogenic nature of the alloy (Fig. S6d), and OC starts to be readable at negative control after day 9 (Fig. S6c), according to differentiation of osteogenic cells. For unknown reasons, OC has not been detected on TNZS alloy, so additional tests are eventually required. For HUVEC (Fig. S7) CD31 and vWf expressions are clear on negative control, eventually decreasing in time as when there are too much cells in vitro, one can observe a decrease of cell activity. Both these factors are however more important for angiogenesis rather than for osteogenesis.

Overall, it could be seen that both TNZS and Ti–6Al–4V alloys have exhibited a good HOC colonization on the substrate, whereas for the latter proliferation rate is more modest when looking to negative control. For HUVEC a little growth was seen on Ti–6Al–4V but much more substantial growth on TNZS. Major osteogenic markers were equivalent or higher on TNZS compared to negative control, which shows it to be a positive candidate for orthopedic applications.

In summary, this study highlights the electrochemical corrosion and biological behaviors of a novel TNZS alloy for orthopedic applications. The electrochemical measurements studied the effects of immersion time and simulated biological media on the corrosion performance of TNZS, Ti–6Al–4V, and CP-Ti biomaterials. Invasive orthopedic implantation provokes inflammatory and severe inflammatory responses that produce acidic environments with H2O2 and biomolecules substances near the metallic biomaterial. The inflammatory and severe inflammatory media were mimicked by additions of H2O2 and H2O2, BSA, and CLH to the PBS solution, respectively. OCP test showed that the values increased with immersion time for all specimens and TNZS alloy had a nobler potential compared others.

The TNZS alloy showed the highest corrosion resistance in all media and electrochemical testing time, based on EIS and PDP tests’ results. The high corrosion resistance was attributed to the presence of silicide phases in the microstructure of TNZS alloy, which have high stability in acidic environments containing H2O2. SEM/EDS analyses after the PDP test proved the synergistic effects of BSA and CLH on the corrosion response of titanium biomaterials in severe inflammatory conditions. The TNZS alloy also exhibits excellent biological activity in terms of HOC and HUVEC proliferation and growth, which makes it a promising biomedical material for orthopedic applications.

The TNZS alloy was prepared from Ti, Nb, Zr, and Si raw materials (Goodfellow Cambridge Ltd., Huntington, UK) with vacuum arc-melting in copper molds, chill cast into steel mold making a rod-like specimen of 20 mm diameter and approximately 100 mm in length. The rod was further wire-cut into specimens without further heat treatment. The starting point of the alloy composition was based on Zr/Nb weight ratios closer to unity, similarly to commercial biomedical alloy TNZ (UNS R58103 by ASTM F1713), with addition of up to 2 wt% Si. The rationale for silicon content was to trigger formation of fine silicides but preserving major metallic matrix, as was calculated with thermodynamic equilibrium as shown below. The chemical composition of the TNZS alloy was determined by inductively coupled plasma optical emission spectrometry (ICP-OES) as 11.15 ± 0.19 wt %Zr, 9.61 ± 0.32 wt %Nb, 1.23 ± 0.20 wt %Si, and Ti as the balance. Commercial purity titanium (CP-Ti, 99.6% purity) and Ti–6Al–4V (Al = 5.5–6.76% wt, V = 3.5–4.5% wt, and Ti = balance) alloy were obtained from Goodfellow Cambridge Ltd. (Huntingdon, UK). Samples were cut into discs 0.5 mm in height and 15 mm in diameter. The surface was ground with 240–2500 grit SiC sandpapers, polished with alumina suspension (0.3 μm, Allied, Canada), then ultrasonically washed with 70% ethanol (Merck, Germany) and deionized water.

The microstructures of the polished TNZS alloy were observed through an optical microscope (OM, Leica DM2500, Germany), and a scanning electron microscope (SEM, TESCAN Mira 3, The Czech Republic) equipped with an energy dispersive spectrometer (EDS, QUANTA-450FEI, USA) after chemical etching with Kroll’s reagent (1 ml HF + 2 ml HNO3 + 100 ml H2O) at room temperature for 5 min. The phase composition of the polished TNZS alloy was identified using X-ray diffractometer (XRD, Philips PW 3040/60, The Netherlands) operated at 30 mA and 40 kV by a monochromatic CuKα as a radiation source with 2θ ranging from 20° to 80°.

Thermodynamic analysis of the TNZS alloy has been made with CALPHAD method81. For the solution phases, liquid, b-phase (BCC structure) and a-phase (HCP structure) substitutional solution model (Ti,Nb,Zr,Si) was used to describe their Gibbs energy functions according to the Redlich–Kister formalism81. Binary and ternary interaction parameters are obtained from the corresponding sub-systems from own thermodynamic database using FACTSage software (FACTSage 8.0, GTT, Germany). For the silicide phases two sublattice models in the format of (Ti, Nb, Zr)aSib were applied and in the first sublattice of this model (metal-rich), binary interaction parameters among the elements are estimated by using solubility data in ternary systems but without the ternary parameter. Although the descriptions of the three silicon-contained ternary subsystems have still uncertainties and require further investigations, recent analysis is resulting in more reliable data in the metal-rich side as the ternary Ti–Nb–Zr system has been sufficiently well described82. In this work thermodynamic description was deployed only to estimate the expected phases within the composition range of the TNZS alloy.

Electrochemical measurements were performed with potentiostat (IviumStat.h standard, The Netherlands) in a conventional three-electrode system with a graphite rod as the counter electrode, Ag/AgCl as a reference electrode (R.E.), and Ti-based specimens as a working electrode (W.E.) with exposed area of 1 cm2 (Fig. S8 in the Supplementary Data file). The five phosphate-buffered saline tablets (PBS, Sigma-Aldrich, USA) were dissolved in 1 l deionized water to prepare the basic solution that refers to the normal physiological condition. One tablet dissolved in 200 ml of deionized water yields PBS solution (0.137 M sodium chloride, 0.0027 M potassium chloride, and 0.01 M phosphate buffer), with pH 7.34 at 25 °C. Reagents H2O2 (30%, Merck, Germany) and hydrochloric acid (HCl; 37%, Merck, Germany) were added to the PBS-based solution to simulate inflammatory conditions. For the severe inflammatory condition H2O2, HCl, bovine serum albumin (BSA, Sigma-Aldrich, Germany) and calcium l-lactate hydrate (CLH, Sigma-Aldrich, Germany) were all added to the PBS-based solution (Supplementary Table ST6). The pH values and conductivity of the solutions were measured using a Metrohm 691 pH meter, and Mettler Toledo Inlab 730 probe, respectively. The measurements were carried out at 37 ± 0.5 °C and under slight anaerobic conditions by purging air + 5% CO2 gas through the solution.

All specimens were immersed in simulated solutions before measurements for 1 and 12 h to evaluate the time-dependent corrosion behavior and formed passive layers stability. The specimens’ open circuit potential (OCP) was recorded during 600 s following each immersion time. Potentiodynamic polarization (PDP) measure–ments were done with a sweep range from − 1.5 to 1.5 V versus OCP voltage at a constant sweep rate of 1 mV·s−1. The electrochemical impedance spectroscopy (EIS) was performed in the frequency range of 100 kHz–0.010 Hz under AC voltage of 10 mV in amplitude around OCP potential. EIS data were examined by the ZView (version 3.1, Scribner Associates) software. The data fitting quality was confirmed by chi-squared (χ2) values that are in the order of 10−3. The reproducibility and reliability of the obtained results have been guaranteed by conducting all tests in triplicate. As a post-corrosion study, the top-view morphology of surfaces exposed to simulated solutions for 12 h was characterized using SEM/EDS after PDP tests.

Biological reactions of the new alloy in comparison with standard titanium alloy Grade 5 (Ti–6Al–4V) were analyzed with human cells (HOC) arising from bone marrow (6th passage) and human endothelial cells (HUVEC; 9th passage, PromoCell) at an independent laboratory for analysis of implantable materials (Laboratoire d’Evaluation des Matériels Implantables—LEMI, Martillac, France) with the details of the protocol as presented earlier83. Briefly, study of the HOC and HUVEC adhesion was analyzed with Live/Dead viability kit where live cells are distinguished by the presence of ubiquitous intracellular esterase activity, determined by the enzymatic conversion of the non-fluorescent cell-permeable calcein AM to the intensely fluorescent calcein (green). Nuclei of dead cells are stained by passive transport of ethidium bromide (red).

Cells proliferation was analyzed (LEMI, Matrillac, France) by seeding cells at 10 K (HOC) or 20 K cells·cm−2 (HUVEC) in triplicate and counted using a hemocytometer after various incubation times (1, 3, 9, 15 and 27 days at 37 °C in a humidified atmosphere containing 5% vol CO2). Samples were covered up first with complete culture medium and incubated at 37 °C during 3 × 30 min to allow serum proteins to absorb on the surface first to mimic in vivo situation following implantation (adsorbed protein layer). Cell counting was performed at the end of the incubation period by detaching cells with using 0.2% wt trypsin in Hank’s balanced Ca/Mg-free solution incubated (2 min) with 0.2% wt Trypan blue in 0.15 M NaCl and counting dead (blue) and living cells (uncolored) with a hemocytometer. Plastic standard PS culture wells were used everywhere as a negative control and Ti–6Al–4V alloy specimens as a comparison material. The analysis of cytoskeleton and focal adhesion formation after 24 h incubation was made through actin, tubulin, vinculin and integrin αvβ3 study with the coating/substrate using immunofluorescence with monoclonals conjugated to fluorescein isothiocyanate isomer I (FITC) or crystalline tetramethyl rhodamine isothiocyanate (TRITC) for vinculin. A nuclear labelling was performed using Hoescht staining83. Study of HOC and HUVEC differentiation was carried out at 1, 3, 9, 15 and 27 days of incubation times with cells seeding at 100 K cells·cm−2. Total RNA was isolated from the cells after 1 and 3 days of contact, cDNA was synthesized and PCR is performed to analyze the genes with a semi-quantitative method, normalizing results to a housekeeping gene (β-actine). Specific genes were related to HOC (collagen type 1 (ColI), alkaline phosphatase (ALP), osteocalcine (OC), Cbfa (core-binding factor subunit alpha-1, also known as Runt-related transcription factor 2 (RUNX2) which is a protein key transcription factor associated with osteoblast differentiation), and for HUVEC (CD31—a platelet endothelial cell adhesion molecule (PECAM-1) and von Willebrand factor (vWf)), with further protocol details as reported previously83.

Image analysis of the results was made with ImageJ (v. 1.53s, NIH, USA) applied on fluorescent images after color thresholding (Huang method in HSV space with green color as the target) to exclude dark background and integrated particle analysis macro; additionally the coverage of the area by the living cells was compared to controls.

The data used to support the findings of this study are available from the corresponding author upon request.

Badv, M., Bayat, F., Weitz, J. I. & Didar, T. F. Single and multi-functional coating strategies for enhancing the biocompatibility and tissue integration of blood-contacting medical implants. Biomaterials 258, 120291 (2020).

Article CAS PubMed Google Scholar

Matusiewicz, H. Potential release of in vivo trace metals from metallic medical implants in the human body: From ions to nanoparticles—A systematic analytical review. Acta Biomater. 10(6), 2379–2403 (2014).

Article CAS PubMed Google Scholar

Niinomi, M. Design and development of metallic biomaterials with biological and mechanical biocompatibility. J. Biomed. Mater. Res. A 107(5), 944–954 (2019).

Article CAS PubMed Google Scholar

Feng, F. et al. Surface characteristics and biocompatibility of ultrafine-grain Ti after sandblasting and acid etching for dental implants. ACS Biomater. Sci. Eng. 5(10), 5107–5115 (2019).

Article CAS PubMed Google Scholar

Shah, F. A., Trobos, M., Thomsen, P. & Palmquist, A. Commercially pure titanium (cp-Ti) versus titanium alloy (Ti6Al4V) materials as bone anchored implants—Is one truly better than the other? Mater. Sci. Eng. C 62, 960–966 (2016).

Article CAS Google Scholar

Costa, B. C. et al. Vanadium ionic species from degradation of Ti–6Al–4V metallic implants: In vitro cytotoxicity and speciation evaluation. Mater. Sci. Eng. C 96, 730–739 (2019).

Article CAS Google Scholar

Kaur, M. & Singh, K. Review on titanium and titanium based alloys as biomaterials for orthopaedic applications. Mater. Sci. Eng. C 102, 844–862 (2019).

Article CAS Google Scholar

Cochis, A., Azzimonti, B., Chiesa, R., Rimondini, L. & Gasik, M. Metallurgical gallium additions to titanium alloys demonstrate a strong time-increasing antibacterial activity without any cellular toxicity. ACS Biomater. Sci. Eng. 5(6), 2815–2820 (2019).

Article CAS PubMed Google Scholar

Ozaltin, K. A. D. I. R. et al. Microstructure and texture evolutions of biomedical Ti–13Nb–13Zr alloy processed by hydrostatic extrusion. Metall. Mater. Trans. A 48, 5747–5755 (2017).

Article CAS Google Scholar

Mazigi, O., Kannan, M. B., Xu, J., Choe, H. C. & Ye, Q. Biocompatibility and degradation of a low elastic modulus Ti–35Nb–3Zr alloy: Nanosurface engineering for enhanced degradation resistance. ACS Biomater. Sci. Eng. 3(4), 509–517 (2017).

Article CAS PubMed Google Scholar

Pérez, D. A. G. et al. Severe plastic deformation and different surface treatments on the biocompatible Ti13Nb13Zr and Ti35Nb7Zr5Ta alloys: Microstructural and phase evolutions, mechanical properties, and bioactivity analysis. J. Alloys Compd. 812, 152116 (2020).

Article Google Scholar

Kumar, P., Mahobia, G. S., Mandal, S., Singh, V. & Chattopadhyay, K. Enhanced corrosion resistance of the surface modified Ti–13Nb–13Zr alloy by ultrasonic shot peening. Corros. Sci. 189, 109597 (2021).

Article CAS Google Scholar

Nielsen, F. H. Update on the possible nutritional importance of silicon. J. Trace. Elem. Med. Biol. 28(4), 379–382 (2014).

Article CAS PubMed Google Scholar

Zhao, X. et al. Enhanced osseointegration of titanium implants by surface modification with silicon-doped titania nanotubes. Int. J. Nanomed. 15, 8583 (2020).

Article CAS Google Scholar

Cravedi, P. et al. Regenerative immunology: The immunological reaction to biomaterials. Transplant Int. 30(12), 1199–1208 (2017).

Article Google Scholar

Sadtler, K. et al. Design, clinical translation and immunological response of biomaterials in regenerative medicine. Nat. Rev. Mater. 1(7), 1–17 (2016).

Article Google Scholar

Wu, J. et al. Moderated crevice corrosion susceptibility of Ti6Al4V implant material due to albumin-corrosion interaction. J. Mater. Sci. Technol. 109, 209–220 (2022).

Article Google Scholar

Bryan, N. et al. The innate oxygen dependant immune pathway as a sensitive parameter to predict the performance of biological graft materials. Biomaterials 33(27), 6380–6392 (2012).

Article MathSciNet CAS PubMed Google Scholar

Franz, S., Rammelt, S., Scharnweber, D. & Simon, J. C. Immune responses to implants—A review of the implications for the design of immunomodulatory biomaterials. Biomaterials 32(28), 6692–6709 (2011).

Article CAS PubMed Google Scholar

Otake, S. et al. Regulation of the expression and activity of glucose and lactic acid metabolism-related genes by protein kinase C in skeletal muscle cells. Biol. Pharm. Bull. 36(9), 1435–1439 (2013).

Article CAS PubMed Google Scholar

Carlander, U. et al. Macrophage-assisted dissolution of gold nanoparticles. ACS Appl. Biol. Mater. 2(3), 1006–1016 (2019).

Article CAS Google Scholar

Fonseca, C. & Barbosa, M. A. Corrosion behaviour of titanium in biofluids containing H2O2 studied by electrochemical impedance spectroscopy. Corros. Sci. 43(3), 547–559 (2001).

Article CAS Google Scholar

Prestat, M., Vucko, F., Holzer, L. & Thierry, D. Microstructural aspects of Ti6Al4V degradation in H2O2-containing phosphate buffered saline. Corros. Sci. 190, 109640 (2021).

Article CAS Google Scholar

Fonseca-García, A. et al. The effect of simulated inflammatory conditions on the surface properties of titanium and stainless steel and their importance as biomaterials. Mater. Sci. Eng. C 66, 119–129 (2016).

Article Google Scholar

Bearinger, J. P., Orme, C. A. & Gilbert, J. L. Effect of hydrogen peroxide on titanium surfaces: In situ imaging and step: Polarization impedance spectroscopy of commercially pure titanium and titanium, 6% aluminum, 4% vanadium. J. Biomed. Mater. Res. A 67(3), 702–712 (2003).

Article PubMed Google Scholar

Yu, F., Addison, O. & Davenport, A. J. A synergistic effect of albumin and H2O2 accelerates corrosion of Ti6Al4V. Acta Biomater. 26, 355–365 (2015).

Article CAS PubMed Google Scholar

Zhang, Y. et al. Time-dependent enhanced corrosion of Ti6Al4V in the presence of H2O2 and albumin. Sci. Rep. 8(1), 1–11 (2018).

ADS Google Scholar

Mabilleau, G. et al. Influence of fluoride, hydrogen peroxide and lactic acid on the corrosion resistance of commercially pure titanium. Acta Biomater. 2(1), 121–129 (2006).

Article CAS PubMed Google Scholar

Benea, L. & Simionescu-Bogatu, N. Reactivity and corrosion behaviors of Ti6Al4V alloy implant biomaterial under metabolic perturbation conditions in physiological solutions. Materials 14(23), 7404 (2021).

Article ADS CAS PubMed PubMed Central Google Scholar

Xing, Z., Lu, C., Hu, D., Miclau, T. III. & Marcucio, R. S. Rejuvenation of the inflammatory system stimulates fracture repair in aged mice. J. Orthop. Res. 28(8), 1000–1006 (2010).

Article CAS PubMed PubMed Central Google Scholar

Mombelli, A. & Cionca, N. Systemic diseases affecting osseointegration therapy. Clin. Oral Implants Res. 17, 97–103 (2006).

Article PubMed Google Scholar

Mazur, A. V., Gasik, M. M. & Mazur, V. I. Microstructure formation in Ti–Si composite subjected to high temperature gradients. Int. J. Mater. Res. 96, 377–379 (2005).

CAS Google Scholar

Li, C., Zhan, Y. & Jiang, W. β-type Ti–Mo–Si ternary alloys designed for biomedical applications. Mater. Des. 34, 479–482 (2012).

Article CAS Google Scholar

Tavares, A. M. G. et al. The addition of Si to the Ti–35Nb alloy and its effect on the corrosion resistance, when applied to biomedical materials. J. Alloys Compd. 591, 91–99 (2014).

Article CAS Google Scholar

Brooks, E. K., Brooks, R. P. & Ehrensberger, M. T. Effects of simulated inflammation on the corrosion of 316L stainless steel. Mater. Sci. Eng. C 71, 200–205 (2017).

Article CAS Google Scholar

Bordbar-Khiabani, A., Bahrampour, S., Mozafari, M. & Gasik, M. Surface functionalization of anodized tantalum with Mn3O4 nanoparticles for effective corrosion protection in simulated inflammatory condition. Ceram. Int. 48, 3148–3156 (2022).

Article CAS Google Scholar

Liu, X. et al. Exploring the biodegradation of pure Zn under simulated inflammatory condition. Corros. Sci. 189, 109606 (2021).

Article CAS Google Scholar

Bordbar-Khiabani, A., Yarmand, B., Sharifi-Asl, S. & Mozafari, M. Improved corrosion performance of biodegradable magnesium in simulated inflammatory condition via drug-loaded plasma electrolytic oxidation coatings. Mater. Chem. Phys. 239, 122003 (2020).

Article CAS Google Scholar

Zhang, Y., Davenport, A. J., Burke, B., Vyas, N. & Addison, O. Effect of Zr addition on the corrosion of Ti in acidic and reactive oxygen species (ROS)-containing environments. ACS Biomater. Sci. Eng. 4(3), 1103–1111 (2018).

Article CAS PubMed Google Scholar

Izquierdo, J. et al. Electrochemical behaviour of ZrTi alloys in artificial physiological solution simulating in vitro inflammatory conditions. Appl. Surf. Sci. 313, 259–266 (2014).

Article CAS Google Scholar

Dargusch, M. S. et al. Comparison of the microstructure and biocorrosion properties of additively manufactured and conventionally fabricated near β Ti–25Nb–3Zr–3Mo–2Sn alloy. ACS Biomater. Sci. Eng. 5(11), 5844–5856 (2019).

Article CAS PubMed Google Scholar

Caha, I. et al. Degradation behavior of Ti–Nb alloys: Corrosion behavior through 21 days of immersion and tribocorrosion behavior against alumina. Corros. Sci. 167, 108488 (2020).

Article CAS Google Scholar

Sotniczuk, A., Kuczyńska-Zemła, D., Kwaśniak, P., Thomas, M. & Garbacz, H. Corrosion behavior of Ti-29Nb-13Ta-4.6Zr and commercially pure Ti under simulated inflammatory conditions—Comparative effect of grain refinement and non-toxic β phase stabilizers. Electrochim. Acta 312, 369–379 (2019).

Article CAS Google Scholar

Engvall, P. & Lundström, I. Physico-chemical considerations of titanium as a biomaterial. Clin. Mater. 9(2), 115–134 (1992).

Article Google Scholar

Tengvall, P., Elwing, H. & Lundström, I. Titanium gel made from metallic titanium and hydrogen peroxide. J. Colloid Interface Sci. 130(2), 405–413 (1989).

Article ADS CAS Google Scholar

Hedberg, Y. S. et al. Surface modified Ti6Al4V for enhanced bone bonding ability—Effects of silver and corrosivity at simulated physiological conditions from a corrosion and metal release perspective. Corros. Sci. 168, 108566 (2020).

Article CAS Google Scholar

Dragus, L., Benea, L., Simionescu, N., Ravoiu, A. & Neaga, V. Effect of the inflammatory conditions and albumin presence on the corrosion behavior of grade 5 Titanium alloy in saliva biological solution. IOP Conf. Ser. Mater. Sci. Eng. 572(1), 012005 (2019).

Article CAS Google Scholar

Gopal, V. & Manivasagam, G. Wear-Corrosion synergistic effect on Ti–6Al–4V alloy in H2O2 and albumin environment. J. Alloys Compd. 830, 154539 (2020).

Article CAS Google Scholar

Fattah-Alhosseini, A., Ansari, A. R., Mazaheri, Y. & Keshavarz, M. K. Effect of immersion time on the passive and electrochemical response of annealed and nano-grained commercial pure titanium in Ringer’s physiological solution at 37° C. Mater. Sci. Eng. C 71, 771–779 (2017).

Article CAS Google Scholar

Hirschorn, B. et al. Determination of effective capacitance and film thickness from constant-phase-element parameters. Electrochim. Acta 55, 6218–6227 (2010).

Article CAS Google Scholar

Pan, J., Thierry, D. & Leygraf, C. Hydrogen peroxide toward enhanced oxide growth on titanium in PBS solution: Blue coloration and clinical relevance. J. Biomed. Mater. Res. 30, 393–402 (1996).

3.0.CO;2-L" data-track-action="article reference" href="https://doi.org/10.1002%2F%28SICI%291097-4636%28199603%2930%3A3%3C393%3A%3AAID-JBM14%3E3.0.CO%3B2-L" aria-label="Article reference 51" data-doi="10.1002/(SICI)1097-4636(199603)30:33.0.CO;2-L">Article CAS PubMed Google Scholar

Venkateswarlu, K. et al. Role of electrolyte chemistry on electronic and in vitro electrochemical properties of micro-arc oxidized titania films on Cp Ti. Electrochim. Acta 105, 468–480 (2013).

Article CAS Google Scholar

Pan, J., Thierry, D. & Leygraf, C. Electrochemical impedance spectroscopy study of the passive oxide film on titanium for implant application. Electrochim. Acta 41, 1143–1153 (1996).

Article CAS Google Scholar

Pan, J., Thierry, D. & Leygraf, C. Electrochemical and XPS studies of titanium for biomaterial applications with respect to the effect of hydrogen peroxide. J. Biomed. Mater. Res. 28, 113–122 (1994).

Article CAS PubMed Google Scholar

Berbel, L. O., Banczek, E. D. P., Karousis, I. K., Kotsakis, G. A. & Costa, I. Determinants of corrosion resistance of Ti–6Al–4V alloy dental implants in an In Vitro model of peri-implant inflammation. PLoS ONE 14, e0210530 (2019).

Article CAS PubMed PubMed Central Google Scholar

Wang, J. L. et al. A closer look at the in vitro electrochemical characterisation of titanium alloys for biomedical applications using in-situ methods. Acta Biomater. 54, 469–478 (2017).

Article ADS CAS PubMed Google Scholar

Qu, Q. et al. Corrosion behavior of titanium in artificial saliva by lactic acid. Materials 7, 5528–5542 (2014).

Article ADS PubMed PubMed Central Google Scholar

Koike, M. & Fujii, H. The corrosion resistance of pure titanium in organic acids. Biomaterials 22, 2931–2936 (2001).

Article CAS PubMed Google Scholar

Caha, I. et al. Interactions between wear and corrosion on cast and sintered Ti–12Nb alloy in comparison with the commercial Ti–6Al–4V alloy. Corros. Sci. 176, 108925 (2020).

Article CAS Google Scholar

Song, Q. T. & Xu, J. (TiZrNbTa)90Mo10 high-entropy alloy: Electrochemical behavior and passive film characterization under exposure to Ringer’s solution. Corros. Sci. 167, 108513 (2020).

Article CAS Google Scholar

Fattah-Alhosseini, A., Imantalab, O. & Ansari, G. The role of grain refinement and film formation potential on the electrochemical behavior of commercial pure titanium in Hank’s physiological solution. Mater. Sci. Eng. C 71, 827–834 (2017).

Article CAS Google Scholar

Zhang, X. L., Jiang, Z. H., Yao, Z. P., Song, Y. & Wu, Z. D. Effects of scan rate on the potentiodynamic polarization curve obtained to determine the Tafel slopes and corrosion current density. Corros. Sci. 51(3), 581–587 (2009).

Article CAS Google Scholar

Cheng, S. et al. Microbially influenced corrosion of stainless steel by marine bacterium Vibrio natriegens: (I) Corrosion behavior. Mater. Sci. Eng. C 29(3), 751–755 (2009).

Article CAS Google Scholar

Al-Mobarak, N. A., Al-Mayouf, A. M. & Al-Swayih, A. A. The effect of hydrogen peroxide on the electrochemical behavior of Ti and some of its alloys for dental applications. Mater. Chem. Phys. 99, 333–340 (2006).

Article CAS Google Scholar

Höhn, S. & Virtanen, S. Effect of inflammatory conditions and H2O2 on bare and coated Ti–6Al–4V surfaces: Corrosion behavior, metal ion release and Ca-P formation under long-term immersion in DMEM. Appl. Surf. Sci. 357, 101–111 (2015).

Article ADS Google Scholar

Narayanan, T. S., Kim, J. & Park, H. W. High performance corrosion and wear resistant Ti–6Al–4V alloy by the hybrid treatment method. Appl. Surf. Sci. 504, 144388 (2020).

Article Google Scholar

Li, R. et al. Crystallization and magnetic properties of [(Fe, Co)0.75Si0.05B0.20]94Nb6 metallic glasses. J. Phys. D Appl. Phys. 42(8), 085006 (2009).

Article ADS Google Scholar

Stoica, M. et al. FeCoBSiNb bulk metallic glasses with Cu additions. Phys. Status Solidi C 7(5), 1331–1335 (2010).

Article ADS CAS Google Scholar

Ram, S. & Johari, G. P. Glass–liquid transition in hyperquenched metal alloys. Philos. Mag. B 61(3), 299–310 (1990).

Article ADS CAS Google Scholar

Karan, T., Ram, S., Stoica, M. & Eckert, J. Effect of copper additives on irreversible melting in [{(Fe0.5Co0.5)0.75B0.2Si0.05}96Nb4]100-xCux, x≤ 3, alloys. Int. J. Nanosci. 10(04), 1013–1017 (2011).

Article CAS Google Scholar

Jiang, Z., Dai, X. & Middleton, H. Effect of silicon on corrosion resistance of Ti–Si alloys. Mater. Sci. Eng. B 176, 79–86 (2011).

Article CAS Google Scholar

Wu, J. et al. Development of biomedical Ti–Nb–Zr–Mn alloys with enhanced mechanical properties and corrosion resistance. Mater. Today Commun. 30, 103027 (2022).

Article CAS Google Scholar

Chui, P., Jing, R., Zhang, F., Li, J. & Feng, T. Mechanical properties and corrosion behavior of β-type Ti–Zr–Nb–Mo alloys for biomedical application. J. Alloys Compd. 842, 155693 (2020).

Article CAS Google Scholar

Cordeiro, J. M. et al. Functionalization of an experimental Ti–Nb–Zr–Ta alloy with a biomimetic coating produced by plasma electrolytic oxidation. J. Alloys Compd. 770, 1038–1048 (2019).

Article CAS Google Scholar

Li, G. et al. Microstructure and corrosion resistance of powder metallurgical Ti–Nb–Zr–Mg alloys with low modulus for biomedical application. Mater. Charact. 192, 112223 (2022).

Article CAS Google Scholar

Salas, L. et al. Tribocorrosion and corrosion behavior of quaternary Ti–24Nb–xZr–ySn alloys in SBF. Mater. Lett. 283, 128903 (2021).

Article CAS Google Scholar

Metikos-Huković, M., Kwokal, A. & Piljac, J. The influence of niobium and vanadium on passivity of titanium-based implants in physiological solution. Biomaterials 24(21), 3765–3775 (2003).

Article PubMed Google Scholar

Hoppe, V. et al. Assessment of mechanical, chemical, and biological properties of Ti–Nb–Zr alloy for medical applications. Materials 14(1), 126 (2020).

Article ADS PubMed PubMed Central Google Scholar

Lu, X., Zhang, T., Lv, Y., Zhang, X. & Dong, Z. Corrosion behviour of micro-arc oxidized titanium in NaCl solution with H2O2 and albumin. Mater. Chem. Phys. 276, 125376 (2022).

Article CAS Google Scholar

Jandová, V. et al. Corrosion behavior of titanium silicide surface with hydrogen peroxide: Formation of sub-μm TiOx-based spheres, nanocomposite TiOx/SiOx phases, and mesoporous TiOx/SiOx network. Appl. Surf. Sci. 529, 147133 (2020).

Article Google Scholar

Kaufman, L. & Bernstein, H. Computer Calculation of Phase Diagrams (Academic, 1970).

Google Scholar

Kumar, K. H., Wollants, P. & Delaey, L. Thermodynamic calculation of Nb–Ti–V phase diagram. Calphad 18, 79 (1994).

Google Scholar

Gasik, M. et al. Reduction of biofilm infection risks and promotion of osteointegration for optimized surfaces of titanium implants. Adv. Healthcare Mater. 1, 117–127 (2012).

Article CAS Google Scholar

Download references

Financial supports of the European Commission under FP6 SME integrated project “Meddelcoat” (contract 026501-2) and from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie ITN “Premurosa” (GA 860462) are gratefully acknowledged. Authors are greatly thankful for the late Dr. A. V. Mazur for help in TNZS alloy preparation, Dr. H. Yu for assistance in thermodynamic database optimization and Dr. D. Sukhomlinov for his help in performing the SEM/EDS. The LEMI assistance in analyzing details for the cells-materials interactions, led by Mrs. Prof. M.-F. Harmand and Mrs. D. Pierron, is also gratefully acknowledged.

Department of Chemical and Metallurgical Engineering, School of Chemical Engineering, Aalto University Foundation, 02150, Espoo, Finland

Aydin Bordbar-Khiabani & Michael Gasik

You can also search for this author in PubMed Google Scholar

You can also search for this author in PubMed Google Scholar

A.B.-K. methodology, writing-original draft, investigation; M.G. funding acquisition, conceptualization, methodology, supervision, writing-review and editing. All authors have read and agreed to the published version of the manuscript.

Correspondence to Aydin Bordbar-Khiabani.

The authors declare no competing interests.

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and Permissions

Bordbar-Khiabani, A., Gasik, M. Electrochemical and biological characterization of Ti–Nb–Zr–Si alloy for orthopedic applications. Sci Rep 13, 2312 (2023). https://doi.org/10.1038/s41598-023-29553-5

Download citation

Received: 02 December 2022

Accepted: 06 February 2023

Published: 09 February 2023

DOI: https://doi.org/10.1038/s41598-023-29553-5

Anyone you share the following link with will be able to read this content:

Sorry, a shareable link is not currently available for this article.

Provided by the Springer Nature SharedIt content-sharing initiative

Scientific Reports (2023)

Chemistry Africa (2023)

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.